Gene Delivery to Mammalian Cells

Publisher : Humana Press

ISBN-13 : 9781617373534

Page : 566 pages

Rating : 4.5/5 from 534 voters

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

More Books:

Gene Delivery to Mammalian Cells
Language: en
Pages: 566
Authors: William C. Heiser
Categories: Science
Type: BOOK - Published: 2010-11-10 - Publisher: Humana Press

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA i
Gene Transfer and Expression in Mammalian Cells
Language: en
Pages: 730
Authors: S.C. Makrides
Categories: Science
Type: BOOK - Published: 2003-10-24 - Publisher: Gulf Professional Publishing

This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors
Gene Delivery to Mammalian Cells
Language: en
Pages: 566
Authors: William C. Heiser
Categories: Science
Type: BOOK - Published: 2008-02-02 - Publisher: Springer Science & Business Media

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA i
Gene Therapy
Language: en
Pages: 370
Authors: Chunsheng Kang
Categories: Medical
Type: BOOK - Published: 2011-06-22 - Publisher: BoD – Books on Demand

The aim of this book is to cover key aspects of existing problems in the field of development and future perspectives in gene therapy. Contributions consist of
Gene Transfer to Animal Cells
Language: en
Pages: 256
Authors: Richard Twyman
Categories: Science
Type: BOOK - Published: 2004-04-01 - Publisher: Garland Science

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitu
Nucleic Acid Transfection
Language: en
Pages: 316
Authors: Wolfgang Bielke
Categories: Science
Type: BOOK - Published: 2010-10-21 - Publisher: Springer Science & Business Media

Gene Delivery into Mammalian Cells: An Overview on Existing Approaches Employed In Vitro and In Vivo, by Peter Hahn and Elizabeth Scanlan * Strategies for the P
Cell Engineering
Language: en
Pages: 223
Authors: Mohamed Al-Rubeai
Categories: Science
Type: BOOK - Published: 2012-12-06 - Publisher: Springer Science & Business Media

The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infec
Biolistic DNA Delivery
Language: en
Pages: 432
Authors: Stephan Sudowe
Categories: Medical
Type: BOOK - Published: 2016-05-01 - Publisher: Humana Press

Another key addition to Springer s Methods in Molecular Biology series, this volume includes methods for direct biolistic transfection of DNA using ballistic de
Advanced Delivery and Therapeutic Applications of RNAi
Language: en
Pages: 536
Authors: Kun Cheng
Categories: Science
Type: BOOK - Published: 2013-04-19 - Publisher: John Wiley & Sons

Commonly used by researchers to develop technologies for modifying and studying genetic process, RNA interference (RNAi) has many potential uses in medicine, bi
Molecular and Cell Biology of Human Gene Therapeutics
Language: en
Pages: 403
Authors: George Dickson
Categories: Science
Type: BOOK - Published: 1995-05-31 - Publisher: Springer

advanced metastatic disease of solid tumors, dictates that each tumor mass, indeed each individual metastasis, will have a unique antigen and cytokine environme